Hotspot

rbcL

LSC · 58952-60379 · 1428 bp

Back to Pedinomonas

Pi 0.49

MarkerSeek score 80.30

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 583
pi: 0.49
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TAGTTTGTGTGGAAAGCCCC	ACTGGTTCGCCTAAAACGTT	50.0	45.0	58.0	58.0	2568 bp	0.5	59.6
rbcL_p2	TAGTTTGTGTGGAAAGCCCC	TGGTTCGCCTAAAACGTTGA	50.0	45.0	58.0	58.0	2566 bp	0.5	59.5
rbcL_p3	ACACACACACAGTCTGTTCC	ACTGGTTCGCCTAAAACGTT	50.0	45.0	58.0	58.0	2493 bp	0.5	59.5
rbcL_p4	ACACACACACAGTCTGTTCC	TGGTTCGCCTAAAACGTTGA	50.0	45.0	58.0	58.0	2491 bp	0.5	59.4
rbcL_p5	TGGCTACTTGCTCACGTTTT	ACTGGTTCGCCTAAAACGTT	45.0	45.0	58.0	58.0	2787 bp	0.5	59.4