Hotspot

orf205

LSC · 56361-56978 · 618 bp

Back to Pedinomonas

Pi 0.473

MarkerSeek score 77.90

Primer available yes

Label orf205

Feature Metadata

label_name: orf205
feature_type: gene
region: LSC
length_bp: 618
variable_sites: 219
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf205_p1	CAGTGAGCGGATAAGCAGAG	CACAAATCACTTGCGACCAC	55.0	50.0	58.1	58.0	1546 bp	0.5	58.5
orf205_p2	CAGTGAGCGGATAAGCAGAG	TCACAAATCACTTGCGACCA	55.0	45.0	58.1	58.0	1547 bp	0.5	58.5
orf205_p3	CGGTCAGTAGGTCAGGAGAA	CACAAATCACTTGCGACCAC	55.0	50.0	58.2	58.0	1631 bp	0.5	58.4
orf205_p4	TCTTCCGGTCAGTAGGTCAG	CACAAATCACTTGCGACCAC	55.0	50.0	58.2	58.0	1636 bp	0.5	58.4
orf205_p5	CAGTGAGCGGATAAGCAGAG	GTAACTGTTCCGCGAAAAGC	55.0	50.0	58.1	58.1	1503 bp	0.5	58.4