Hotspot

atpB

LSC · 60738-62180 · 1443 bp

Back to Pedinomonas

Pi 0.495

MarkerSeek score 78.60

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1443
variable_sites: 550
pi: 0.495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	AACTGGTTCGATGTCGTAGC	CCAGACGGCGAATAATACGT	50.0	50.0	58.0	57.9	2777 bp	0.5	58.9
atpB_p2	CTGGTTCGATGTCGTAGCAA	CCAGACGGCGAATAATACGT	50.0	50.0	58.0	57.9	2775 bp	0.5	58.9
atpB_p3	AACTGGTTCGATGTCGTAGC	AGCACGCCATTCTTGTTTTG	50.0	45.0	58.0	57.9	2376 bp	0.5	58.8
atpB_p4	AACTGGTTCGATGTCGTAGC	CAGCACGCCATTCTTGTTTT	50.0	45.0	58.0	57.9	2377 bp	0.5	58.8
atpB_p5	CTGGTTCGATGTCGTAGCAA	AGCACGCCATTCTTGTTTTG	50.0	45.0	58.0	57.9	2374 bp	0.5	58.8