Hotspot

accD

LSC · 51037-52182 · 1146 bp

Back to Pedinomonas

Pi 0.178

MarkerSeek score 81.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1146
variable_sites: 199
pi: 0.178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CTGGATTCGAACCAGCGTAA	TGCAACTTCCATGGCTTCTT	50.0	45.0	58.0	58.0	2119 bp	0.5	60.0
accD_p2	TGGATTCGAACCAGCGTAAG	TGCAACTTCCATGGCTTCTT	50.0	45.0	58.0	58.0	2118 bp	0.5	60.0
accD_p3	CATCCCCATTAACCACTCGG	TGCAACTTCCATGGCTTCTT	55.0	45.0	58.0	58.0	2076 bp	0.5	59.7
accD_p4	CCATCCCCATTAACCACTCG	TGCAACTTCCATGGCTTCTT	55.0	45.0	58.0	58.0	2077 bp	0.5	59.7
accD_p5	TGGATTCGAACCAGCGTAAG	ACGAACCCGAACCAATTTCA	50.0	45.0	58.0	58.0	1606 bp	0.5	59.7