Hotspot

trnE-UUC-trnT-GGU

LSC · 30935-31572 · 638 bp

Back to Pedicularis

Pi 0.054

MarkerSeek score 85.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 125
pi: 0.054
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AAACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	59.1	57.9	620-710 bp	1.0	82.4
trnE-UUC-trnT-GGU_p2	AACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	57.9	50.0	58.4	57.9	619-709 bp	1.0	80.0
trnE-UUC-trnT-GGU_p3	CCTAAACCACTAGACGATGGG	TACCGCTGAGTTAAAAGGGC	52.4	50.0	57.5	57.9	623-713 bp	1.0	79.5
trnE-UUC-trnT-GGU_p4	CTAAACCACTAGACGATGGGG	TACCGCTGAGTTAAAAGGGC	52.4	50.0	57.5	57.9	622-712 bp	1.0	79.5
trnE-UUC-trnT-GGU_p5	GCCTCCTTGAAAGAGAGATGT	TACCGCTGAGTTAAAAGGGC	47.6	50.0	57.4	57.9	644-734 bp	1.0	78.2