Hotspot

trnS(gcu)-rrf

IRa · 202407-203155 · 749 bp

Back to Pediastrum

Pi 0.218

MarkerSeek score 85.30

Primer available yes

Label trnS(gcu)-rrf

Feature Metadata

label_name: trnS(gcu)-rrf
feature_type: igs
region: IRa
length_bp: 749
variable_sites: 114
pi: 0.218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(gcu)-rrf_p1	CGGATTAGCAATCAGTCGCT	GTTGGAAGTCTGGCAGGAAA	50.0	50.0	58.1	58.0	735-832 bp	1.0	90.7
trnS(gcu)-rrf_p2	CGGATTAGCAATCAGTCGCT	TGGAAGTCTGGCAGGAAAAC	50.0	50.0	58.1	58.0	733-830 bp	1.0	90.7
trnS(gcu)-rrf_p3	CGGATTAGCAATCAGTCGCT	ACTTGTTGGAAGTCTGGCAG	50.0	50.0	58.1	58.0	739-836 bp	1.0	90.6
trnS(gcu)-rrf_p4	CGGATTAGCAATCAGTCGCT	GACTTGTTGGAAGTCTGGCA	50.0	50.0	58.1	58.0	740-837 bp	1.0	90.6
trnS(gcu)-rrf_p5	TACGGAGAGAGAGGGATTCG	TGGAAGTCTGGCAGGAAAAC	55.0	50.0	57.7	58.0	781-878 bp	1.0	89.8