Hotspot

ycf1-trnS(gcu)

IRa · 201391-202317 · 927 bp

Back to Pediastrum

Pi 0.233

MarkerSeek score 90.50

Primer available yes

Label ycf1-trnS(gcu)

Feature Metadata

label_name: ycf1-trnS(gcu)
feature_type: igs
region: IRa
length_bp: 927
variable_sites: 201
pi: 0.233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 90.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnS(gcu)_p1	TGTTCCAGCTCCAAAATCGT	TAGGAGAGATGGCTGAGTGG	45.0	55.0	57.7	57.9	1138-1941 bp	1.0	88.6
ycf1-trnS(gcu)_p2	TGTTCCAGCTCCAAAATCGT	AGCGACTGATTGCTAATCCG	45.0	50.0	57.7	58.1	1113-1916 bp	1.0	88.1
ycf1-trnS(gcu)_p3	TGTTCCAGCTCCAAAATCGT	AAGCAGTAGGAGAGATGGCT	45.0	50.0	57.7	57.8	1144-1947 bp	1.0	87.8
ycf1-trnS(gcu)_p4	TGTTCCAGCTCCAAAATCGT	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.7	58.2	1070-1873 bp	1.0	87.6
ycf1-trnS(gcu)_p5	TGTTCCAGCTCCAAAATCGT	TTTTTCGTACCGAGGGTTCG	45.0	50.0	57.7	58.2	1083-1886 bp	1.0	87.6