Hotspot

rrl-rrf

IRb · 118431-118663 · 233 bp

Back to Pediastrum

Pi 0.325

MarkerSeek score 89.30

Primer available yes

Label rrl-rrf

Feature Metadata

label_name: rrl-rrf
feature_type: igs
region: IRb
length_bp: 233
variable_sites: 68
pi: 0.325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 89.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrl-rrf_p1	TTTCCTCAAGATGAGCGCTC	GTTTTCCTGCCAGACTTCCA	50.0	50.0	58.0	58.0	458-465 bp	1.0	91.7
rrl-rrf_p2	TTTCCTCAAGATGAGCGCTC	TTTCCTGCCAGACTTCCAAC	50.0	50.0	58.0	58.0	456-463 bp	1.0	91.7
rrl-rrf_p3	CTTTCCTCAAGATGAGCGCT	GTTTTCCTGCCAGACTTCCA	50.0	50.0	58.0	58.0	459-466 bp	1.0	91.7
rrl-rrf_p4	CTTTCCTCAAGATGAGCGCT	TTTCCTGCCAGACTTCCAAC	50.0	50.0	58.0	58.0	457-464 bp	1.0	91.7
rrl-rrf_p5	TTTCCTCAAGATGAGCGCTC	CTGCCAGACTTCCAACAAGT	50.0	50.0	58.0	58.0	452-459 bp	1.0	91.7