Hotspot

trnD(guc)-rps11

LSC · 97014-97671 · 658 bp

Back to Pediastrum

Pi 0.297
MarkerSeek score 85.20
Primer available yes
Label trnD(guc)-rps11

Feature Metadata

label_name
trnD(guc)-rps11
feature_type
igs
region
LSC
length_bp
658
variable_sites
184
pi
0.297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD(guc)-rps11_p1 GGGCAGTGCTCTAACCAATT AAGACGTGCTTATCGTGGTC 50.0 50.0 58.2 58.0 1043-1976 bp 1.0 88.7
trnD(guc)-rps11_p2 GGGCAGTGCTCTAACCAATT GACGTGCTTATCGTGGTCTT 50.0 50.0 58.2 58.0 1041-1974 bp 1.0 88.7
trnD(guc)-rps11_p3 TAACTACGGGATTGACGGGA AAGACGTGCTTATCGTGGTC 50.0 50.0 57.9 58.0 1090 bp 0.5 58.8
trnD(guc)-rps11_p4 TAACTACGGGATTGACGGGA GAAGACGTGCTTATCGTGGT 50.0 50.0 57.9 58.0 1091 bp 0.5 58.8
trnD(guc)-rps11_p5 TAACTACGGGATTGACGGGA GACGTGCTTATCGTGGTCTT 50.0 50.0 57.9 58.0 1088 bp 0.5 58.8