Hotspot

trnH(gug)-trnS(uga)

LSC · 94453-95238 · 786 bp

Back to Pediastrum

Pi 0.377
MarkerSeek score 89.10
Primer available yes
Label trnH(gug)-trnS(uga)

Feature Metadata

label_name
trnH(gug)-trnS(uga)
feature_type
igs
region
LSC
length_bp
786
variable_sites
280
pi
0.377
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
89.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(gug)-trnS(uga)_p1 AAGGCAGTGGATTGTGACTC AAAGGAAAGGTGGCAGAGTG 50.0 50.0 57.8 58.0 929-1515 bp 1.0 89.2
trnH(gug)-trnS(uga)_p2 TGGTAAGGCAGTGGATTGTG AAAGGAAAGGTGGCAGAGTG 50.0 50.0 57.8 58.0 933-1519 bp 1.0 89.1
trnH(gug)-trnS(uga)_p3 GTGGTAAGGCAGTGGATTGT AAAGGAAAGGTGGCAGAGTG 50.0 50.0 57.8 58.0 934-1520 bp 1.0 89.1
trnH(gug)-trnS(uga)_p4 AAGGCAGTGGATTGTGACTC GGTTCGAATCCCTCCCTTTC 50.0 55.0 57.8 58.0 861-1447 bp 1.0 89.1
trnH(gug)-trnS(uga)_p5 AAGGCAGTGGATTGTGACTC GTTCGAATCCCTCCCTTTCC 50.0 55.0 57.8 58.0 860-1446 bp 1.0 89.1