Hotspot

rpoBb-rpoBa

LSC · 60526-62367 · 1842 bp

Back to Pediastrum

Pi 0.234

MarkerSeek score 81.30

Primer available yes

Label rpoBb-rpoBa

Feature Metadata

label_name: rpoBb-rpoBa
feature_type: igs
region: LSC
length_bp: 1842
variable_sites: 332
pi: 0.234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoBb-rpoBa_p1	CCTTCTTGAACCCAGTCACC	TGTATGTTAGTGGATCAAAAATTCT	55.0	28.0	58.1	55.0	1915-2204 bp	1.0	52.0
rpoBb-rpoBa_p2	CCTTCTTGAACCCAGTCACC	TGTTAGTGGATCAAAAATTCTTT	55.0	26.1	58.1	52.8	1911-2200 bp	1.0	52.0
rpoBb-rpoBa_p3	CCTTCTTGAACCCAGTCACC	TGTATGTTAGTGGATCAAAAATTCTT	55.0	26.9	58.1	55.7	1915-2204 bp	1.0	52.0
rpoBb-rpoBa_p4	CCTTCTTGAACCCAGTCACC	TTGTATGTTAGTGGATCAAAAATTCT	55.0	26.9	58.1	55.7	1916-2205 bp	1.0	52.0
rpoBb-rpoBa_p5	CCTTCTTGAACCCAGTCACC	TGTATGTTAGTGGATCAAAAATTC	55.0	29.2	58.1	53.6	1915-2204 bp	1.0	52.0