Hotspot

ftsH-trnT(ugu)

LSC · 35328-36721 · 1394 bp

Back to Pediastrum

Pi 0.282

MarkerSeek score 76.60

Primer available yes

Label ftsH-trnT(ugu)

Feature Metadata

label_name: ftsH-trnT(ugu)
feature_type: igs
region: LSC
length_bp: 1394
variable_sites: 276
pi: 0.282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH-trnT(ugu)_p1	GCTTCAGTTCGGTTGCTTTC	AGCCTGCTTAACTCAATCGG	50.0	50.0	58.0	58.0	1794 bp	0.5	59.5
ftsH-trnT(ugu)_p2	GCTTCAGTTCGGTTGCTTTC	GAAGAACCAACTGCGCATTC	50.0	50.0	58.0	58.0	2366 bp	0.5	59.4
ftsH-trnT(ugu)_p3	GGAGCAAGAACCCGAATGAT	AGCCTGCTTAACTCAATCGG	50.0	50.0	58.0	58.0	1684 bp	0.5	59.4
ftsH-trnT(ugu)_p4	GGAGCAAGAACCCGAATGAT	GAAGAACCAACTGCGCATTC	50.0	50.0	58.0	58.0	2256 bp	0.5	59.4
ftsH-trnT(ugu)_p5	GCTTCAGTTCGGTTGCTTTC	GAGTTTTTCTTCGGCTTCGC	50.0	50.0	58.0	58.0	1908 bp	0.5	59.3