Hotspot

rpl32-rps2

LSC · 4228-6005 · 1778 bp

Back to Pediastrum

Pi 0.277

MarkerSeek score 72.50

Primer available yes

Label rpl32-rps2

Feature Metadata

label_name: rpl32-rps2
feature_type: igs
region: LSC
length_bp: 1778
variable_sites: 258
pi: 0.277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.52
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-rps2_p1	TTTCGTGGAGCAGCTTCTTT	GATTGTTTTCCTTCGCCTGC	45.0	50.0	58.0	58.0	2464 bp	0.5	59.6
rpl32-rps2_p2	TCGTGGAGCAGCTTCTTTAC	GATTGTTTTCCTTCGCCTGC	50.0	50.0	57.9	58.0	2462 bp	0.5	59.2
rpl32-rps2_p3	CGTGGAGCAGCTTCTTTACT	GATTGTTTTCCTTCGCCTGC	50.0	50.0	57.9	58.0	2461 bp	0.5	59.2
rpl32-rps2_p4	TTGAAGCCAAGATTGTCGCT	GATTGTTTTCCTTCGCCTGC	45.0	50.0	58.1	58.0	2101 bp	0.5	59.0
rpl32-rps2_p5	CGTGTAAATGCAGCAACTCG	GATTGTTTTCCTTCGCCTGC	50.0	50.0	58.1	58.0	2344 bp	0.5	58.8