Hotspot

DR496_pgp011

LSC · 15345-16268 · 924 bp

Back to Pediastrum

Pi 0.354

MarkerSeek score 77.60

Primer available yes

Label DR496_pgp011

Feature Metadata

label_name: DR496_pgp011
feature_type: gene
region: LSC
length_bp: 924
variable_sites: 240
pi: 0.354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR496_pgp011_p1	TCTGCCAAAATGGAAGGCTT	GAAGCACCGAACCGAAAAAG	45.0	50.0	58.0	58.0	1372 bp	0.5	59.6
DR496_pgp011_p2	ATTGCGCTGTCCACAAATTG	GAAGCACCGAACCGAAAAAG	45.0	50.0	57.9	58.0	1796 bp	0.5	59.2
DR496_pgp011_p3	TCTGCCAAAATGGAAGGCTT	CGATCGAAGATTGTCGCTCT	45.0	50.0	58.0	57.9	1598 bp	0.5	59.0
DR496_pgp011_p4	TCTGCCAAAATGGAAGGCTT	GCCAAAGCGAAAAACTTCGA	45.0	45.0	58.0	57.9	1673 bp	0.5	59.0
DR496_pgp011_p5	TCTGCCAAAATGGAAGGCTT	CCGAACCGAAAAAGCGAATC	45.0	50.0	58.0	58.1	1366 bp	0.5	58.9