Hotspot

rpl16

LSC · 80887-82512 · 1626 bp

Back to Pecteilis

Pi 0.008

MarkerSeek score 54.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1626
variable_sites: 13
pi: 0.008
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGGGCACTGCTTCTTTGATT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	58.0	58.0	2211-2215 bp	1.0	81.2
rpl16_p2	TTGGGCACTGCTTCTTTGAT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	58.0	58.0	2212-2216 bp	1.0	81.2
rpl16_p3	GAGATAAAGAAACCCGCGGT	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	58.0	58.0	2006-2010 bp	1.0	81.1
rpl16_p4	CGAGCAATCGCACCAAAAAT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	58.0	58.0	2377-2381 bp	1.0	80.9
rpl16_p5	CGCACCAAAAATCCGAGTTC	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	58.0	58.0	2369-2373 bp	1.0	80.8