Hotspot

clpP1

LSC · 68717-70996 · 2280 bp

Back to Pecteilis

Pi 0.0101

MarkerSeek score 50.90

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2280
variable_sites: 23
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	ATAGAGCGGGTTGGCTTAGA	CGAACAATAGGCCGATCCAA	50.0	50.0	58.2	58.1	2787-2805 bp	1.0	79.4
clpP1_p2	TAGAGCGGGTTGGCTTAGAT	CGAACAATAGGCCGATCCAA	50.0	50.0	58.2	58.1	2786-2804 bp	1.0	79.4
clpP1_p3	CGGGTTGGCTTAGATCGATC	CGAACAATAGGCCGATCCAA	55.0	50.0	58.2	58.1	2781-2799 bp	1.0	79.4
clpP1_p4	TGGCTGTCTTGCGTTTCTAA	CGAACAATAGGCCGATCCAA	45.0	50.0	57.8	58.1	2860-2878 bp	1.0	79.1
clpP1_p5	TTGGCTGTCTTGCGTTTCTA	CGAACAATAGGCCGATCCAA	45.0	50.0	57.8	58.1	2861-2879 bp	1.0	79.1