Hotspot

trnR-ACG-rrn5

IRa · 129413-129663 · 251 bp

Back to Pecteilis

Pi 0.00797
MarkerSeek score 53.20
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
251
variable_sites
2
pi
0.00797
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 370 bp 1.0 77.7
trnR-ACG-rrn5_p2 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 373 bp 1.0 77.7
trnR-ACG-rrn5_p3 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 371 bp 1.0 77.7
trnR-ACG-rrn5_p4 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 374 bp 1.0 77.7
trnR-ACG-rrn5_p5 CCACGTGCTCTAATCCTCTG ATTCTGGTGTCCTAGGCGTA 55.0 50.0 57.8 57.8 403 bp 1.0 77.4