Hotspot

ndhC-trnV-UAC

LSC · 49605-50780 · 1176 bp

Back to Pecteilis

Pi 0.0111

MarkerSeek score 65.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1176
variable_sites: 13
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TTTTCTGGCCCCTCCCTAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.3	58.1	1331-1416 bp	1.0	77.7
ndhC-trnV-UAC_p2	TTTCTGGCCCCTCCCTAATC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.8	58.1	1330-1415 bp	1.0	74.0
ndhC-trnV-UAC_p3	TTTTCTGGCCCCTCCCTAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.3	58.7	1316-1401 bp	1.0	73.1
ndhC-trnV-UAC_p4	CTTTTCTGGCCCCTCCCTAA	CCGAGAAAGTCTACGGTTCG	55.0	55.0	59.0	58.1	1332-1417 bp	1.0	72.2
ndhC-trnV-UAC_p5	TTCTGGCCCCTCCCTAATC	CCGAGAAAGTCTACGGTTCG	57.9	55.0	58.1	58.1	1329-1414 bp	1.0	71.8