Hotspot

rps14-psaB

LSC · 36522-36655 · 134 bp

Back to Pecteilis

Pi 0.0373

MarkerSeek score 74.00

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 5
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	GTTCCAATTTCTGGCGCTTC	ACTTACGCGGCTTTCTTGAT	50.0	45.0	58.0	57.9	234 bp	1.0	80.8
rps14-psaB_p2	GTTCCAATTTCTGGCGCTTC	ATTCACTTACGCGGCTTTCT	50.0	45.0	58.0	57.9	238 bp	1.0	80.8
rps14-psaB_p3	GTTCCAATTTCTGGCGCTTC	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.0	58.1	303 bp	1.0	80.5
rps14-psaB_p4	GTTCCAATTTCTGGCGCTTC	TGGATTAGCCCACTTTTCCG	50.0	50.0	58.0	57.9	268 bp	1.0	80.4
rps14-psaB_p5	GTTCCAATTTCTGGCGCTTC	GGATTAGCCCACTTTTCCGT	50.0	50.0	58.0	57.9	267 bp	1.0	80.4