Hotspot

atpH-atpI

LSC · 12932-13668 · 737 bp

Back to Pecteilis

Pi 0.0125

MarkerSeek score 68.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 9
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	855-856 bp	1.0	80.7
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	834-835 bp	1.0	80.2
atpH-atpI_p3	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.8	57.9	854-855 bp	1.0	80.1
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	833-834 bp	1.0	79.7
atpH-atpI_p5	CCAGGTCCGATAGAAGCAAG	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.8	57.9	860-861 bp	1.0	79.5