Hotspot

ycf1

SSC · 124435-130017 · 5583 bp

Back to Paulownia

Pi 0.0018

MarkerSeek score 27.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5583
variable_sites: 28
pi: 0.0018
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCATTTCTGACAGCCCCG	TCGATTTACAGAGACAGGCT	50.0	45.0	58.5	56.0	245 bp	1.0	61.1
ycf1_p2	TTTCATTTCTGACAGCCCCG	TTCGATTTACAGAGACAGGCT	50.0	42.9	58.5	56.8	246 bp	1.0	59.3
ycf1_p3	TTTCATTTCTGACAGCCCCG	TTTCGATTTACAGAGACAGGCT	50.0	40.9	58.5	57.5	247 bp	1.0	56.9
ycf1_p4	TTTCATTTCTGACAGCCCCG	TTCGATTTACAGAGACAGGC	50.0	45.0	58.5	55.2	246 bp	1.0	54.9
ycf1_p5	TTCATTTCTGACAGCCCCG	TCGATTTACAGAGACAGGCT	52.6	45.0	57.8	56.0	244 bp	1.0	54.9