Hotspot

rpl22

LSC · 84614-85072 · 459 bp

Back to Paulownia

Pi 0.00148

MarkerSeek score 28.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 459
variable_sites: 2
pi: 0.00148
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTTGTGCAAACCAAAGGGAA	TGATTCATTAGTAGGAGATAAACCT	45.0	32.0	57.3	55.0	545 bp	1.0	40.3
rpl22_p2	TGTGCAAACCAAAGGGAATG	TGATTCATTAGTAGGAGATAAACCT	45.0	32.0	57.1	55.0	543 bp	1.0	40.3
rpl22_p3	GTTGTGCAAACCAAAGGGAA	TGATTCATTAGTAGGAGATAAACCTT	45.0	30.8	57.3	55.7	545 bp	1.0	40.3
rpl22_p4	GTTGTGCAAACCAAAGGGAA	TGATTCATTAGTAGGAGATAAACC	45.0	33.3	57.3	53.7	545 bp	1.0	40.3
rpl22_p5	CCAAGTCTGAAACCAAGTGGA	TGATTCATTAGTAGGAGATAAACCT	47.6	32.0	58.1	55.0	507 bp	1.0	40.3