Hotspot

psbT-psbN

LSC · 75626-75685 · 60 bp

Back to Paulownia

Pi 0.0375

MarkerSeek score 60.60

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 4
pi: 0.0375
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	ATCCATTCGAAGAACACGGG	47.6	50.0	57.6	58.0	125-126 bp	1.0	71.2
psbT-psbN_p2	TCGAGAACCACCTAAAGTTCC	ATCCATTCGAAGAACACGGG	47.6	50.0	57.3	58.0	126-127 bp	1.0	69.0
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	174-175 bp	1.0	68.4
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	47.6	50.0	57.6	57.6	182-183 bp	1.0	67.6
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	47.6	50.0	57.6	57.6	185-186 bp	1.0	67.5