Hotspot

accD-psaI

LSC · 59929-60656 · 728 bp

Back to Paulownia

Pi 0.00755

MarkerSeek score 54.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 12
pi: 0.00755
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	890-911 bp	1.0	82.9
accD-psaI_p2	TAAGCTCCACGCCTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	824-845 bp	1.0	82.8
accD-psaI_p3	CTCCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	820-841 bp	1.0	82.7
accD-psaI_p4	CCAGAAGGGCTTATTCGACC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	896-917 bp	1.0	82.2
accD-psaI_p5	TAAGCTCCACGCCTTCTTTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	830-851 bp	1.0	82.1