Hotspot

trnG(GCC)-tRNA-fM(CAU)

LSC · 37017-37201 · 185 bp

Back to Paulownia

Pi 0.0147

MarkerSeek score 62.80

Primer available yes

Label trnG(GCC)-tRNA-fM(CAU)

Feature Metadata

label_name: trnG(GCC)-tRNA-fM(CAU)
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 7
pi: 0.0147
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(GCC)-tRNA-fM(CAU)_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	243-251 bp	1.0	84.1
trnG(GCC)-tRNA-fM(CAU)_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	244-252 bp	1.0	84.0
trnG(GCC)-tRNA-fM(CAU)_p3	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	279-287 bp	1.0	83.6
trnG(GCC)-tRNA-fM(CAU)_p4	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	237-245 bp	1.0	83.6
trnG(GCC)-tRNA-fM(CAU)_p5	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	280-288 bp	1.0	83.5