Hotspot

trnR(UCU)-atpA

LSC · 10421-10528 · 108 bp

Back to Paulownia

Pi 0.0112

MarkerSeek score 48.10

Primer available yes

Label trnR(UCU)-atpA

Feature Metadata

label_name: trnR(UCU)-atpA
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 3
pi: 0.0112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(UCU)-atpA_p1	AAAAATCGGAATGAACGGCG	TTCAGGAACAAATGGACCGG	45.0	50.0	57.7	58.1	241-245 bp	1.0	78.5
trnR(UCU)-atpA_p2	GAACGGCGTCCATTGTCTAA	TTCAGGAACAAATGGACCGG	50.0	50.0	58.3	58.1	229-233 bp	1.0	78.4
trnR(UCU)-atpA_p3	ACGGCGTCCATTGTCTAATG	TTCAGGAACAAATGGACCGG	50.0	50.0	58.3	58.1	227-231 bp	1.0	77.9
trnR(UCU)-atpA_p4	AACGGCGTCCATTGTCTAAT	TTCAGGAACAAATGGACCGG	45.0	50.0	57.6	58.1	228-232 bp	1.0	77.5
trnR(UCU)-atpA_p5	AAAAATCGGAATGAACGGCG	AGGAACAAATGGACCGGTTT	45.0	45.0	57.7	57.6	238-242 bp	1.0	76.3