Hotspot

trnS(GCU)-trnG(UCC)

LSC · 8767-9402 · 636 bp

Back to Paulownia

Pi 0.00612

MarkerSeek score 53.00

Primer available yes

Label trnS(GCU)-trnG(UCC)

Feature Metadata

label_name: trnS(GCU)-trnG(UCC)
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 9
pi: 0.00612
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnG(UCC)_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	689-705 bp	1.0	55.1
trnS(GCU)-trnG(UCC)_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	688-704 bp	1.0	55.1
trnS(GCU)-trnG(UCC)_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	692-708 bp	1.0	54.0
trnS(GCU)-trnG(UCC)_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	691-707 bp	1.0	54.0
trnS(GCU)-trnG(UCC)_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	690-706 bp	1.0	54.0