Hotspot

trnH(GUG)-psbA

LSC · 77-564 · 488 bp

Back to Paulownia

Pi 0.0142
MarkerSeek score 58.10
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
488
variable_sites
15
pi
0.0142
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.91
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 374-567 bp 1.0 84.8
trnH(GUG)-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 388-581 bp 1.0 84.7
trnH(GUG)-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 387-580 bp 1.0 84.7
trnH(GUG)-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 389-582 bp 1.0 84.7
trnH(GUG)-psbA_p5 GATCCACTTGGCTACATCCG TGGAAGTTATGCACGAACGT 55.0 45.0 58.1 57.8 406-599 bp 1.0 84.0