Hotspot

ycf1

SSC · 123839-128652 · 4814 bp

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Pi 0.024

MarkerSeek score 56.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4814
variable_sites: 298
pi: 0.024
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCTGCGAGAGTTCTGTTAC	TCTCCTTGAAACCTTGGTGC	55.0	50.0	58.0	58.0	283-337 bp	0.857143	82.4
ycf1_p2	CCCTGCGAGAGTTCTGTTAC	AAGAAGCCTGGGAAAACCTG	55.0	50.0	58.0	58.0	562-616 bp	0.857143	80.7
ycf1_p3	CCCTGCGAGAGTTCTGTTAC	ACGGGAAGAAAGATTACGGC	55.0	50.0	58.0	58.0	591-645 bp	0.857143	80.6
ycf1_p4	TCCCTGCGAGAGTTCTGTTA	AAGAAGCCTGGGAAAACCTG	50.0	50.0	58.1	58.0	563-617 bp	0.857143	80.1
ycf1_p5	CCCTGCGAGAGTTCTGTTAC	TACCAAACGAGGTGTTGGTG	55.0	50.0	58.0	58.0	838-877 bp	0.714286	72.8