Hotspot

clpP

LSC · 72534-73139 · 606 bp

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Pi 0.0388

MarkerSeek score 71.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 70
pi: 0.0388
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	TATTTCCGCGCAAGGATTCA	50.0	45.0	58.0	58.0	1223-2632 bp	1.0	84.2
clpP_p2	CTGAACGAGTCGCACATACA	TGTTATGGACCTCCCCAGTT	50.0	50.0	58.0	57.9	1251-2660 bp	1.0	84.0
clpP_p3	CTGAACGAGTCGCACATACA	ATATGGACAGCAAGCAGTCG	50.0	50.0	58.0	58.1	1396-2805 bp	1.0	83.7
clpP_p4	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	1510-2919 bp	1.0	83.4
clpP_p5	CCGAACCGCATGAGTATGAA	TATTTCCGCGCAAGGATTCA	50.0	45.0	58.1	58.0	1062-2466 bp	0.857143	77.0