Hotspot

accD

LSC · 59437-60528 · 1092 bp

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Pi 0.0495

MarkerSeek score 86.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1092
variable_sites: 125
pi: 0.0495
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAAAAAGGGGTTCATATTCA	ACTCGGAGTCCAATAAACTA	33.3	40.0	53.0	53.1	1135-2137 bp	1.0	48.4
accD_p2	TCAAAAAGGGGTTCATATTCAATCT	ACTCGGAGTCCAATAAACTA	32.0	40.0	57.0	53.1	1135-2137 bp	1.0	48.4
accD_p3	TCAAAAAGGGGTTCATATTCA	ACTCGGAGTCCAATAAACTAA	33.3	38.1	53.0	54.0	1135-2137 bp	1.0	48.4
accD_p4	TCAAAAAGGGGTTCATATTCAATCT	ACTCGGAGTCCAATAAACTAA	32.0	38.1	57.0	54.0	1135-2137 bp	1.0	48.4
accD_p5	TCAAAAAGGGGTTCATATTCAA	ACTCGGAGTCCAATAAACTA	31.8	40.0	53.9	53.1	1135-2137 bp	1.0	48.4