Hotspot

rpl32-trnL-UAG

SSC · 113343-114148 · 806 bp

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Pi 0.0364
MarkerSeek score 77.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
806
variable_sites
63
pi
0.0364
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.95
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTACGACAAACAAATAAGTCA AGCGTGTCTACCGATTTCAC 31.8 50.0 54.0 58.0 811-860 bp 1.0 50.1
rpl32-trnL-UAG_p2 TGTACGACAAACAAATAAGTCA CAGCGTGTCTACCGATTTCA 31.8 50.0 54.0 58.0 812-861 bp 1.0 50.1
rpl32-trnL-UAG_p3 TGTACGACAAACAAATAAGTCA TCCTAAGAGCAGCGTGTCTA 31.8 50.0 54.0 57.9 821-870 bp 1.0 50.0
rpl32-trnL-UAG_p4 TGTACGACAAACAAATAAGTCA CTAAGAGCAGCGTGTCTACC 31.8 55.0 54.0 57.8 819-868 bp 1.0 50.0
rpl32-trnL-UAG_p5 TGTACGACAAACAAATAAGTCA TCTTTTAGAAGACGGCCTGC 31.8 50.0 54.0 57.9 879-928 bp 1.0 49.6