Hotspot

trnN-GUU-ndhF

SSC · 110237-110816 · 580 bp

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Pi 0.0987

MarkerSeek score 78.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 580
variable_sites: 128
pi: 0.0987
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CTACCACTGAGCTACTGAGGA	TAGGAACTGGGAGTGGAAGG	52.4	55.0	58.0	58.0	566-1487 bp	0.428571	59.8
trnN-GUU-ndhF_p2	TCTACCACTGAGCTACTGAGG	TAGGAACTGGGAGTGGAAGG	52.4	55.0	58.0	58.0	567-1488 bp	0.428571	59.8
trnN-GUU-ndhF_p3	CTACCACTGAGCTACTGAGGA	GGGAGTGGAAGGAAGGGTAT	52.4	55.0	58.0	58.1	558-1479 bp	0.428571	59.4
trnN-GUU-ndhF_p4	TCTACCACTGAGCTACTGAGG	GGGAGTGGAAGGAAGGGTAT	52.4	55.0	58.0	58.1	559-1480 bp	0.428571	59.4
trnN-GUU-ndhF_p5	TACCACTGAGCTACTGAGGA	TAGGAACTGGGAGTGGAAGG	50.0	55.0	56.8	58.0	565-1486 bp	0.428571	58.3