Hotspot

accD-psaI

LSC · 60529-61082 · 554 bp

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Pi 0.0345

MarkerSeek score 70.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 55
pi: 0.0345
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTGACAGCCAGTTTTGGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	862-1054 bp	1.0	88.8
accD-psaI_p2	AGTATGGGATCCGTAGTGGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1083-1275 bp	1.0	88.2
accD-psaI_p3	GATTCCTGTAGCAATGGGGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1129-1321 bp	0.857143	81.6
accD-psaI_p4	GGATTCCTGTAGCAATGGGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1130-1322 bp	0.857143	81.6
accD-psaI_p5	GGGATTCCTGTAGCAATGGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1131-1323 bp	0.857143	81.6