Hotspot

atpF-atpH

LSC · 13977-14359 · 383 bp

Back to Patrinia

Pi 0.0234

MarkerSeek score 68.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 25
pi: 0.0234
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	568-574 bp	1.0	77.4
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GGCTTGGTTGTAGCATTAGC	50.0	50.0	57.9	57.1	497-503 bp	1.0	76.9
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGAGGT	50.0	47.6	57.9	58.0	563-569 bp	1.0	75.1
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCTTGGTTGTAGCATTAGCAC	50.0	47.6	57.9	57.9	496-502 bp	1.0	74.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GAGGCAGAGGGAAAAATACGA	50.0	47.6	57.9	57.7	566-572 bp	1.0	73.0