Hotspot

rps16-trnQ-UUG

LSC · 6104-7860 · 1757 bp

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Pi 0.0211
MarkerSeek score 69.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1757
variable_sites
90
pi
0.0211
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.1 57.3 1798-1833 bp 1.0 81.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1825-1860 bp 1.0 81.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGCTATTC 45.0 55.0 58.1 58.9 1816-1851 bp 1.0 79.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTAAGGCAACGGGTTTTGGT 45.0 50.0 58.1 59.0 1826-1861 bp 1.0 78.7
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTGGTAAGGCAACGGGTTTT 45.0 50.0 58.1 59.0 1829-1864 bp 1.0 78.7