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trnH-GUG-psbA

LSC · 164-505 · 342 bp

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Pi 0.0865

MarkerSeek score 61.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 33
pi: 0.0865
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.44
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GCAATGGAACCATAGACGGT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.0	57.9	552-553 bp	0.285714	56.3
trnH-GUG-psbA_p2	GCAATGGAACCATAGACGGT	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	58.0	58.0	1173-1174 bp	0.285714	53.9
trnH-GUG-psbA_p3	GCAATGGAACCATAGACGGT	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	58.0	58.0	1179-1180 bp	0.285714	53.9
trnH-GUG-psbA_p4	GCAATGGAACCATAGACGGT	GGAGCTTAGTTTCCGTCTGG	50.0	55.0	58.0	58.0	1174-1175 bp	0.285714	53.9
trnH-GUG-psbA_p5	GCAATGGAACCATAGACGGT	GGGAGCTTAGTTTCCGTCTG	50.0	55.0	58.0	58.0	1175-1176 bp	0.285714	53.9