Hotspot

rps2

LSC · 14351-15061 · 711 bp

Back to Pastinaca

Pi 0.00281

MarkerSeek score 46.30

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GCGCTCCTGACCAATTAGAA	AGGCCTGAAAGAGAACGTTG	50.0	50.0	58.0	58.1	1782-1786 bp	1.0	79.6
rps2_p2	AAGCGCTCCTGACCAATTAG	AGGCCTGAAAGAGAACGTTG	50.0	50.0	58.0	58.1	1784-1788 bp	1.0	79.6
rps2_p3	ACGTGAGTAAAGCCAAAGCA	AGGCCTGAAAGAGAACGTTG	45.0	50.0	58.0	58.1	1875-1879 bp	1.0	79.5
rps2_p4	GCGCTCCTGACCAATTAGAA	GTACCGGATTCGAGGGATTG	50.0	55.0	58.0	57.8	1738-1742 bp	1.0	79.4
rps2_p5	AAGCGCTCCTGACCAATTAG	GTACCGGATTCGAGGGATTG	50.0	55.0	58.0	57.8	1740-1744 bp	1.0	79.3