Hotspot

rps12-trnV-GAC

IRb · 99470-101375 · 1906 bp

Back to Pastinaca

Pi 0.00271

MarkerSeek score 62.70

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1906
variable_sites: 5
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	GCAACTTTACGTAGGGCAGA	GTCAAGGTGACACTCTACCG	50.0	55.0	57.9	57.7	1922-1985 bp	1.0	79.3
rps12-trnV-GAC_p2	GCAACTTTACGTAGGGCAGA	GGCTCGAACTGATGACTTCC	50.0	55.0	57.9	58.4	1947-2010 bp	1.0	79.2
rps12-trnV-GAC_p3	CGTAGGGCAGAGTTTGGTTT	GTCAAGGTGACACTCTACCG	50.0	55.0	58.1	57.7	1913-1976 bp	1.0	79.2
rps12-trnV-GAC_p4	GCAACTTTACGTAGGGCAGA	GCTCGAACTGATGACTTCCA	50.0	50.0	57.9	57.6	1946-2009 bp	1.0	79.1
rps12-trnV-GAC_p5	CGTAGGGCAGAGTTTGGTTT	GGCTCGAACTGATGACTTCC	50.0	55.0	58.1	58.4	1938-2001 bp	1.0	79.1