Hotspot

trnH-GUG-trnL-CAA

IRb · 93485-94983 · 1499 bp

Back to Pastinaca

Pi 0.0173

MarkerSeek score 63.30

Primer available yes

Label trnH-GUG-trnL-CAA

Feature Metadata

label_name: trnH-GUG-trnL-CAA
feature_type: igs
region: IRb
length_bp: 1499
variable_sites: 25
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-trnL-CAA_p1	CTCAAGGCAGTGGATTGTGA	AAATGGTAGACACGCGAGAC	50.0	50.0	57.8	58.0	1576-1626 bp	1.0	82.2
trnH-GUG-trnL-CAA_p2	CTCAAGGCAGTGGATTGTGA	TGAAATGGTAGACACGCGAG	50.0	50.0	57.8	58.0	1578-1628 bp	1.0	82.2
trnH-GUG-trnL-CAA_p3	CTCAAGGCAGTGGATTGTGA	GCCTTGGTGGTGAAATGGTA	50.0	50.0	57.8	58.1	1588-1638 bp	1.0	81.5
trnH-GUG-trnL-CAA_p4	CTCAAGGCAGTGGATTGTGA	TGGTGAAATGGTAGACACGC	50.0	50.0	57.8	58.2	1581-1631 bp	1.0	80.6
trnH-GUG-trnL-CAA_p5	CTCAAGGCAGTGGATTGTGA	GAAATGGTAGACACGCGAGA	50.0	50.0	57.8	57.7	1577-1627 bp	1.0	80.0