Hotspot

ndhC-trnV-UAC

LSC · 50076-51179 · 1104 bp

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Pi 0.0139
MarkerSeek score 68.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1104
variable_sites
15
pi
0.0139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCAGGCCCTTTGTTAATCGG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1241-1256 bp 1.0 80.5
ndhC-trnV-UAC_p2 TAATCGGGGCTAAAACTCCG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.4 58.1 1228-1243 bp 1.0 76.6
ndhC-trnV-UAC_p3 TCAGGCCCTTTGTTAATCGG GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1226-1241 bp 1.0 75.9
ndhC-trnV-UAC_p4 CCCTTTGTTAATCGGGGCTA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.3 58.1 1236-1251 bp 1.0 75.7
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.8 58.1 1223-1238 bp 1.0 74.9