Hotspot

ycf3-trnS-GGA

LSC · 43924-44744 · 821 bp

Back to Pastinaca

Pi 0.011

MarkerSeek score 62.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 9
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	910 bp	1.0	71.7
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	931 bp	1.0	71.2
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	881 bp	1.0	71.0
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	883 bp	1.0	70.4
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	911 bp	1.0	69.4