Hotspot

trnE-UUC-trnT-GGU

LSC · 30120-30910 · 791 bp

Back to Pastinaca

Pi 0.0166

MarkerSeek score 63.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 13
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	885-895 bp	1.0	80.7
trnE-UUC-trnT-GGU_p2	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	60.0	50.0	58.2	58.0	920-930 bp	1.0	80.2
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	850-860 bp	1.0	80.0
trnE-UUC-trnT-GGU_p4	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	60.0	50.0	58.2	57.9	885-895 bp	1.0	79.5
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	882-892 bp	1.0	79.5