Hotspot

rps2-rpoC2

LSC · 15062-15274 · 213 bp

Back to Pastinaca

Pi 0.0425

MarkerSeek score 64.10

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 9
pi: 0.0425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GATGTTCCAATATCTTTTTGTCA	TCCACGATACGTCAGAACAA	30.4	45.0	53.3	56.6	290-294 bp	1.0	42.7
rps2-rpoC2_p2	GATGTTCCAATATCTTTTTGTCA	TTCCACGATACGTCAGAACA	30.4	45.0	53.3	56.6	291-295 bp	1.0	42.7
rps2-rpoC2_p3	GATGTTCCAATATCTTTTTGTCA	TTCCACGATACGTCAGAACAA	30.4	42.9	53.3	57.4	291-295 bp	1.0	42.7
rps2-rpoC2_p4	GATGTTCCAATATCTTTTTGTCA	TTTCCACGATACGTCAGAACA	30.4	42.9	53.3	57.4	292-296 bp	1.0	42.7
rps2-rpoC2_p5	GATGTTCCAATATCTTTTTGTCAT	TCCACGATACGTCAGAACAA	29.2	45.0	53.9	56.6	290-294 bp	1.0	42.7