Hotspot

rpl32-trnL-UAG

SSC · 112267-113018 · 752 bp

Back to Passiflora

Pi 0.0327
MarkerSeek score 79.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
752
variable_sites
160
pi
0.0327
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAAAGAAAAACCTGGGCTA AGCGTGTCTACCGATTTCAC 35.0 50.0 53.1 58.0 772-831 bp 0.952381 51.1
rpl32-trnL-UAG_p2 AAAAGAAAAACCTGGGCTAA AGCGTGTCTACCGATTTCAC 35.0 50.0 53.1 58.0 771-830 bp 0.952381 51.1
rpl32-trnL-UAG_p3 AAAAAGAAAAACCTGGGCTA CAGCGTGTCTACCGATTTCA 35.0 50.0 53.1 58.0 773-832 bp 0.952381 51.1
rpl32-trnL-UAG_p4 AAAAGAAAAACCTGGGCTAA CAGCGTGTCTACCGATTTCA 35.0 50.0 53.1 58.0 772-831 bp 0.952381 51.1
rpl32-trnL-UAG_p5 AAAAAGAAAAACCTGGGCTAA AGCGTGTCTACCGATTTCAC 33.3 50.0 54.1 58.0 772-831 bp 0.952381 50.4