Hotspot

ndhF-rpl32

SSC · 111564-112092 · 529 bp

Back to Passiflora

Pi 0.0327

MarkerSeek score 79.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 529
variable_sites: 101
pi: 0.0327
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGTAACGAAAGGT	GCTGCCCAAGATCCTTTTCT	45.0	50.0	55.3	58.2	580-689 bp	0.904762	65.6
ndhF-rpl32_p2	AAAGTAGGAGTCCCATTCCT	GCTGCCCAAGATCCTTTTCT	45.0	50.0	55.1	58.2	613-722 bp	0.904762	63.7
ndhF-rpl32_p3	AAAAGTAGGAGTCCCATTCCT	GCTGCCCAAGATCCTTTTCT	42.9	50.0	55.9	58.2	614-723 bp	0.904762	62.5
ndhF-rpl32_p4	AAAAGTAGGAGTCCCATTCC	GCTGCCCAAGATCCTTTTCT	45.0	50.0	54.3	58.2	614-723 bp	0.904762	57.4
ndhF-rpl32_p5	CTGGAAGTGTAACGAAAGGTA	GCTGCCCAAGATCCTTTTCT	42.9	50.0	55.3	58.2	580-689 bp	0.904762	57.3