Hotspot

rps8-rpl14

LSC · 82119-82335 · 217 bp

Back to Passiflora

Pi 0.0326

MarkerSeek score 77.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 217
variable_sites: 56
pi: 0.0326
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATTCCTTTTCCGCCCATGTC	GAGGAATTGAGGCAGGTCAA	50.0	50.0	58.5	57.5	341-363 bp	0.952381	78.4
rps8-rpl14_p2	GATTCCTTTTCCGCCCATGT	GAGGAATTGAGGCAGGTCAA	50.0	50.0	58.5	57.5	342-364 bp	0.952381	78.4
rps8-rpl14_p3	ATTCCTTTTCCGCCCATGTC	TCCAAAGGGAAGTCGCATTT	50.0	45.0	58.5	57.7	378-379 bp	0.095238	37.7
rps8-rpl14_p4	GATTCCTTTTCCGCCCATGT	TCCAAAGGGAAGTCGCATTT	50.0	45.0	58.5	57.7	379-380 bp	0.095238	37.7
rps8-rpl14_p5	GCCCATGTCAGCATTTCGTA	TCCAAAGGGAAGTCGCATTT	50.0	45.0	58.6	57.7	367-368 bp	0.095238	37.0