Hotspot

psbM-petN

LSC · 70961-72346 · 1386 bp

Back to Passiflora

Pi 0.0272

MarkerSeek score 77.80

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1386
variable_sites: 235
pi: 0.0272
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	ATGGGGAAGAAGTGGACTCT	42.9	50.0	57.6	57.6	1118-1522 bp	1.0	77.9
psbM-petN_p2	TGCTACTGCACTGTTCATTC	ATGGGGAAGAAGTGGACTCT	45.0	50.0	56.1	57.6	1118-1522 bp	1.0	73.8
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	TGGGCTGCTTTAATGGTAGTC	42.9	47.6	57.6	58.0	1165-1569 bp	1.0	72.7
psbM-petN_p4	TGCTACTGCACTGTTCATTCT	TGGGGAAGAAGTGGACTCTA	42.9	50.0	57.6	57.0	1117-1521 bp	1.0	72.6
psbM-petN_p5	GCTACTGCACTGTTCATTCT	ATGGGGAAGAAGTGGACTCT	45.0	50.0	55.8	57.6	1117-1521 bp	1.0	71.4