Hotspot

ycf3-psaA

LSC · 56472-57448 · 977 bp

Back to Passiflora

Pi 0.0229

MarkerSeek score 85.60

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 977
variable_sites: 127
pi: 0.0229
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCGCAGAATTGGTTGAAGA	GCCCATTCCTCGAAAGAAGT	45.0	50.0	58.1	57.9	1055-1264 bp	1.0	90.2
ycf3-psaA_p2	TGAAGCGCAGAATTGGTTGA	GCCCATTCCTCGAAAGAAGT	45.0	50.0	58.4	57.9	1058-1267 bp	1.0	87.9
ycf3-psaA_p3	TTGAAGCGCAGAATTGGTTG	GCCCATTCCTCGAAAGAAGT	45.0	50.0	57.6	57.9	1059-1268 bp	1.0	87.7
ycf3-psaA_p4	AGCGCAGAATTGGTTGAAGA	GAAGTTTTGACGGGATCCCT	45.0	50.0	58.1	57.5	1040-1249 bp	1.0	87.2
ycf3-psaA_p5	AGCGCAGAATTGGTTGAAGA	TGTTCTTGAGAAATGGCCGG	45.0	50.0	58.1	58.5	1122-1289 bp	0.952381	82.9