Hotspot

rpoB-clpP

LSC · 25925-26942 · 1018 bp

Back to Passiflora

Pi 0.0441

MarkerSeek score 76.00

Primer available yes

Label rpoB-clpP

Feature Metadata

label_name: rpoB-clpP
feature_type: igs
region: LSC
length_bp: 1018
variable_sites: 198
pi: 0.0441
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-clpP_p1	CACGAGCCCATATTCTTGCT	GGCCAGGATAAAAGATGCCA	50.0	50.0	58.0	57.9	1817-1892 bp	0.809524	82.3
rpoB-clpP_p2	CACGAGCCCATATTCTTGCT	TGTCATCGTTATCGTCGTCG	50.0	50.0	58.0	57.9	1959-2034 bp	0.761905	80.7
rpoB-clpP_p3	AGAGGTTCCCAGGGAATTCA	TGGCGATGTCTTCCTGAATG	50.0	50.0	57.9	58.0	1850-1925 bp	0.714286	79.4
rpoB-clpP_p4	CACGAGCCCATATTCTTGCT	TGGCGATGTCTTCCTGAATG	50.0	50.0	58.0	58.0	2025-2100 bp	0.714286	78.7
rpoB-clpP_p5	CCGGGGCTTTGCAATATTTG	TGGCGATGTCTTCCTGAATG	50.0	50.0	58.1	58.0	1903-1978 bp	0.666667	76.4